LAMTOR5 Knockout cell line (Jurkat)

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LAMTOR5 Knockout cell line (Jurkat) Order

LAMTOR5 Knockout cell line (Jurkat)
Catalog#
YKO-HT10862
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LAMTOR5 Knockout cell line (Jurkat)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
LAMTOR5 Knockout cell line (Jurkat)
Species
Human
Gene Name
LAMTOR5
Gene ID
10542
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LAMTOR5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LAMTOR5 gene knockout LAMTOR5 Knockout cell line (Jurkat) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LAMTOR5 Knockout cell line (Jurkat)
LAMTOR5 Knockout cell line (Jurkat)
Catalog#
YKO-HT10862
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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